Molecular Biology and Nanomedicine

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Clinical Performance and Bonding Mechanism of Three Resin Adhesives

Kun Wu;;

Abstract: Juxtaposed surfaces could be bond to achieve marginal sealing and adhesive of interface between different kinds of substrate. Therefore, the purpose of the current study is to investigate the bonding strength of three common resin adhesives after bonding and polymerization, and to evaluate the bonding effect. PanaviaTM-F resin adhesive, Rely 3M EPSE resin adhesive, and Kerr NX3 resin adhesive were used to prepare modules (10 × 10 × 3 mm3). The glass permeable ceramic was made into a rectangular component (10 × 10 × 10 mm3), ensuring all surfaces to be smooth. Three kinds of different adhesives were bonded to surface of the glass-infiltrated ceramic. Tensile tests, compression tests and shear tests were performed on different adhesives after cold and hot cycles to comprehensively evaluate the differences in the clinical properties of adhesives. After testing, the surface hardness of Kerr NX3 resin adhesive was the highest among the three, and less affected by water storage. The tensile strength and compressive capacity of Kerr NX3 resin were much stronger than those of the other two adhesives. After cold and hot cycles, Kerr NX3 resin was 39% higher than Panavia TM-F resin and 15% higher than Rely 3M EPSE resin. Scanning Electron Microscopy (SEM) observations of morphology and failure surfaces of three adhesives showed that the repairing effect of Kerr NX3 resin was the best and the bonding strength was the highest. Compared with the PanaviaTM-F and Rely 3M EPSE resin, the bonding strength of Kerr NX3 resin was the highest with best repairing effect..

Keywords: bonding strength; compression test; resin bonding; shear force; surface-dimensional hardness, tensile test.

DOI: 10.37813/j.mbn.2707-4692.017

Published on: 2 April 2021

Viewed: 44 / Downloaded: 368 / Cited:0

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Screen and Identify Key Biomarkers Related to Bladder Cancer Through Multi-View Analysis

Xiaxia Wang;Yanrui Ding;

Abstract: Accurate tumor staging is the premise and foundation of precision medicine. This study aims to find the effective biomarkers in the staging process of bladder cancer tumors. First, screen key gene module related to the staging of bladder cancer. Then, taking the hub genes in the key gene module as features, a stage prediction model is constructed by logistic regression and stochastic gradient descent method. It shows that the molecular combination of CTHRC1, COL6A3, TIMP2 and SULF1 can be used as an indicator for stage diagnosis. Importantly, the COL1A1 is not only the pivot gene in the weighted gene co-expression network, but also the core of the protein-protein interaction network. And a set of single-cell RNA sequencing data in Gene Expression Omnibus (GEO) database is used to verify the results. The consistent conclusions indicate that COL1A1 is a potential oncogene and biomarker in the progression of bladder cancer, and can be used as a molecular target for the treatment of bladder cancer to slow the rate of tumor development. In addition, function and pathway enrichment analysis show that focal adhesion is a crucial pathway in the tumor metastasis of bladder cancer, and members of the fibrous collagen family play an important role in the proliferation and spread of tumor cells. The biomarkers and pathways identified in this study provide an important basis for the determination of personalized medical programs..

Keywords: bladder cancer; biomarkers; focal adhesion; tumor staging; COL1A1.

DOI: 10.37813/j.mbn.2707-4692.016

Published on: 29 March 2021

Viewed: 54 / Downloaded: 381 / Cited:0

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Examining Nanoparticle Interactions on a Cellular Scale Using Correlative Light Electron Microscopy

Brian Caffrey;;

Abstract: One key area of nanomedicine research is the mechanism of action of nanoparticles within the body, it’s biodistribution and pharmacokinetic properties. However, insight into the nanoparticle-cell interface is limited, due in part to the heterogeneous nature of nanoparticles as a drug class and the numerous possible interactions between cell and nanoparticle which stem from this diversity such as surface modifications, particle composition and final target tissue. Knowledge of the specific events which occur once a nanotherapeutic has reached its target are still very early in development, this is in part due to the difficulty of resolving interactions in sufficient spatiotemporal resolutions for a concrete understanding of the mechanism for nanoparticle-cellular uptake. However, this is crucially important for our understanding of the therapeutic capabilities of nanoconstructs as a whole. Traditionally, the study of nanoparticle- cell interactions has remained exclusively in either the light or electron microscopies, sacrificing either high resolution cellular information or accurate identification of specific biomolecules through fluorescent markers respectively. This study demonstrates a method for the study and categorization of nanoparticle-cell interactions using Correlative Light Electron Microscopy (CLEM), towards monitoring different drug delivery methods and their effect on target tissues at high resolutions..

Keywords: CLEM; focused ion beam-scanning electron microscopy (FIB-SEM); confocal microscopy; nanoparticle-cell interactions; transferrin-mediated uptake.

DOI: 10.37813/j.mbn.2707-4692.015

Published on: 25 March 2021

Viewed: 103 / Downloaded: 389 / Cited:0

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Role of TGF-β/Smad Signaling Pathway-Related Proteins in Clinicopathological Features and Prognosis and Survival of Patients with Nasopharyngeal Carcinoma

Yanwei Rao;Rui Yang;

Abstract: Nasopharyngeal carcinoma (NPC) is the most common cancer originating in the nasopharynx with high incidence of nasopharyngeal carcinoma. This study aims to identify possible prognostic factors of related proteins in the TGF-β/Smad signaling pathway in NPC. The expression of TGF-β1, TGF-βRI, TGF-βRII, TGF-β2, Smad4, Smad7 and RUNX3 in NPC tissues and nasopharyngitis tissues was detected. Besides, the association of TGF-β/Smad signaling pathway-related protein expressions and prognosis of NPC was analyzed. Initially, the NPC tissues showed higher expression of TGF-β1 and Smad7 and lower expression of Smad4, TGF-βRII, TGF-β2 and RUNX3. Meanwhile, the nonkeratinizing differentiated squamous cell carcinoma showed higher positive expression of TGF-βRI, patients in stage III-IV presented higher positive expression of TGF-β1 and Smad7, and patients with lymph node metastasis showed higher positive expression of TGF-β1 and Smad7. Furthermore, TGF-β1, TGF-β2 and Smad4 were independent factors for the prognosis of NPC. Our study suggests that NPC presents with up-regulated expression of positive expression rates of TGF-β1 and Smad7 and down-regulated TGF-β2, TGF-βRⅡ, Smad4 and RUNX3, and additionally, TGF-β1, TGF-β2 and Smad4 are independent factors for the prognosis of NPC, which could be regarded as the novel target for the treatment of NPC..

Keywords: nasopharyngeal carcinoma; TGF-β; Smad; TGF-β/Smad signaling pathway; clinicopathological features; prognosis.

DOI: 10.37813/j.mbn.2707-4692.014

Published on: 10 March 2020

Viewed: 47 / Downloaded: 345 / Cited:0

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Percutaneous Surgery Provides Better Functional Outcomes for Displaced Intra-Articular Calcaneal Fractures

Zeyong Xie;;

Abstract: We performed a network meta-analysis to compare the effects of different surgical treatments has on displaced intra-articular calcaneal fractures (DIACFs). PubMed, Embase, and Cochrane Library databases were searched for randomized controlled trials (RCTs) from the inception to February 2017. The RCTs providing effects of the different surgical treatments for DIACFs were also included as a significant outlier in this study. A network meta-analysis combining direct and indirect evidence were also used to conduct and evaluate the weighted mean difference (WMD) between odds ratio (OR) value and surface under the cumulative ranking curves (SUCRA) of the different surgical treatments for DIACFs. Fifteen eligible RCTs were acquired and incorporated into this network meta-analysis. In comparison with the traditional open reduction and internal fixation (ORIF), the operating time of the percutaneous surgery was relatively short; the American Orthopaedic Foot and Ankle Society hindfoot scale (AOFAS) of percutaneous surgery and the MOS 36-item Short-Form Health Survey (SF-36) physical component score (PCS) of traditional ORIF and traditional ORIF + primary subtalar arthrodesis observations were all higher than the usual conservative treatment. As far as the operating time, hospitalization, and △bohler's angle go, the effects of percutaneous surgery showed better results than the ones discovered by the traditional ORIF for DIACFs; as for the functional score of AOFAS score, SF-36 (PCS), SF-36 (MCS), and visual analogue scale (VAS), the effects of both percutaneous surgery and traditional ORIF were shown to be relatively better. This network meta-analysis indicated that that the effect of the percutaneous surgery for DIACFs was relatively better..

Keywords: displaced intra-articular calcaneal fractures; effects; surgical treatment; open reduction and internal fixation; network meta-analysis.

DOI: 10.37813/j.mbn.2707-4692.013

Published on: 5 February 2021

Viewed: 58 / Downloaded: 484 / Cited:0

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Qi and Blood Deficiency Contributes to Peripheral T-Cell Dysfunction and Reduced Natural Killer Cell Activity in Gastric Cancer

Zeyong Xie;;

Abstract: Gastric cancer (GC), a major cause of cancer-related mortality, is susceptible to invasion and metastasis, which triggers dismal prognosis. Traditional Chinese medicine (TCM) exerts a pivotal role in various cancers, including GC. Correct syndrome differentiation is the most important principle guiding the treatment strategy. Herein, the present study aims to explore the relationship of different TCM syndrome types of GC patients and the cellular immune function, which was evaluated by observing peripheral T-cell subsets and activity of natural killer (NK) cells. GC patients and healthy controls were enrolled in this study, with venous blood during fasting collected. Patients with deficiency syndromes exhibited decreased number of CD4+ T-cells, ratio of CD4+/CD8+ T-cells and the activity of NK cells as compared with those with sufficiency syndromes. Then, the relationship between TCM syndrome types and TNM classification was analyzed, which revealed that TCM syndrome types were associated with TNM classification. Finally, we evaluated the number of T-cell subsets and NK cell activity in peripheral blood by means of flow cytometry and lactate dehydrogenase (LDH) release assay. GC patients with qi and blood deficiency showed reduced CD4+ T-cells, CD4+/CD8+ T-cells and NK cell activity and increased CD8+ T-cells when compared with other syndrome types, which indicated significantly impaired cellular immune function. Taken together, we concluded that peripheral T-cell subsets and NK cell activity, representing the cellular immune function, were associated with different TCM syndrome types of GC. Notably, qi and blood deficiency led to the most severe cellular immune dysfunction of the GC patients..

Keywords: traditional Chinese medicine syndrome type; gastric cancer; peripheral blood; T-cell subsets; natural killer cell activity; flow cytometry..

DOI: 10.37813/j.mbn.2707-4692.012

Published on: 8 January 2021

Viewed: 83 / Downloaded: 615 / Cited:0

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A Network Meta-Analysis on the Diagnostic Values of MiR-21/MiR-16/MiR-34a/let-7b-5p/let-7g/MiR-218-5p in Non-Small-Cell Lung Cancer

Yifei Yun;Yutong Zhang;

Abstract: Non-small-cell lung cancer (NSCLC) is a major subtype of lung cancer, accompanied by dismal prognosis. Recent studies have reported microRNAs (miRNAs) may act as diagnosis biomarkers in NSCLC. This network meta-analysis was performed to identify miRNA and their potential diagnostic value for NSCLC systematically. Several databases were recruited for eligible studies, and the retrieval spectrum ranged from the inception to June 2018. Besides, 142 NSCLC patients and 168 patients with benign lung disease were enrolled. Direct and indirect evidence were incorporated to evaluate weighted mean difference (WMD) and ranking probability (RP) based on surface under the cumulative ranking curves (SUCRAs). The expressions of miRNAs were further validated by RT-qPCR. The sensitivity, specificity, and receiver operator characteristic (ROC) curve were used to evaluate diagnostic performance. The expression of miR-192 and miR-17 was lower than that of miR-21 in plasma samples of NSCLC. However, the expression of miR-16, miR-34a and miR-1-5p was lower while the levels of let-7g and mR-218-5p were higher than that of miR-21 in NSCLC tissues. miR-21 and let-7g levels were upregulated in plasma/tissues of NSCLS, but miR-16, miR-34a, let-7b-5p and miR-218-5p expression was downregulated. In the analysis of ROC curve, let-7g (sensitivity: 0.901; specificity: 0.893) and miR-218-5p (0.817, 0.964) in plasma as well as miR-16 (0.915, 0.964), miR-34a (0817, 0.964) and miR-218-5p (0.831, 0.964) in tissues revealed higher diagnostic value for NSCLC. It demonstrates that let-7g/miR-218-5p in plasma and miR-16/miR-34a/miR-218-5p in tissues can potentially be used as diagnostic biomarkers of NSCLC..

Keywords: non-small-cell lung cancer; microRNA; case-control study; network meta-analysis.

DOI: 10.37813/j.mbn.2707-4692.011

Published on: 11 December 2020

Viewed: 77 / Downloaded: 693 / Cited:0

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Effects on Embryo Transfer in Patients with Hydrosalpinx Pretreated with Different Treatments: A Network Meta-Analysis

Fang Yang;Jinrong Jin;

Abstract: Hydrosalpinx can affect the success of embryo transfer (ET). We made a network meta-analysis (NMA) to compare the effects of five hydrosalpinx pretreatments of ET, including salpingectomy, hydrosalpinx aspiration, interventional ultrasound sclerotherapy, proximal tubal occlusion and salpingostomy. This study was based on data-set available from the published studies. The relevant studies were retrieved from Cochrane Library, PubMed and Embase from the inception to January 2017. Data was extracted from cohort studies and the combination of direct and indirect evidence was conducted to assess odds ratio (OR) and surface under the cumulative ranking curves (SUCRA) values of the effects of five hydrosalpinx pretreatments of ET. Thirteen eligible cohort studies were included in this NMA. The results of this NMA demonstrated that compared with the control group (without any treatment), salpingectomy and hydrosalpinx aspiration had comparatively higher ongoing pregnancy rate and implantation rate, interventional ultrasound sclerotherapy had comparatively higher implantation rate. In addition, the cluster analysis revealed that salpingectomy and interventional ultrasound sclerotherapy had better effects on ET. Taken together, this NMA suggested that salpingectomy and interventional ultrasound sclerotherapy had better effects on embryo transfer in patients with hydrosalpinx..

Keywords: hydrosalpinx; embryo transfer; effects; cohort study; Bayesian network model.

DOI: 10.37813/j.mbn.2707-4692.010

Published on: 30 October 2020

Viewed: 129 / Downloaded: 728 / Cited:0

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Glutathione S-transferase (GSTT1 and GSTP1) Polymorphisms Improves the Short- and Long-Term Efficacy of Concurrent Chemoradiotherapy in Advanced Ovarian Cancer

Xiao Xu;Zhuo Zhong;

Abstract: Over the past four decades, patients with advanced ovarian cancer (OC) has enjoyed greatly improved survival rate considering advances in combined chemotherapy with platinum and paclitaxel. We investigated the association between polymorphisms within glutathione S-transferase genes (GSTM1, GSTT1 and GSTP1) and short- and long-term efficacy of concurrent chemoradiotherapy (CCRT) in advanced OC. Patients with advanced OC and healthy females were selected in our study. Multiplex polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (PCR-RFLP) were applied to detect homozygous deletions in GSTM1 and GSTT1, as well as codon 105 genetic polymorphisms in GSTP1 exon 5. All the patients with advanced OC were treated with CCRT. Toxicity of chemotherapy, short-term efficacy, recurrence and metastasis, respectively. Long-term survival outcomes were checked with a further examination and all patients were followed up for three years after CCRT. Compared with healthy females, patients with advanced OC showed higher frequencies of GSTT1 non-null genotype and homozygous mutation type GG of GSTP. GSTP1 genetic polymorphisms were relevant to neutropenia resulted from CCRT in patients with advanced OC. Compared with patients with GSTT1 non-null genotype, those with GSTT1 null genotype appeared to enjoy a better short-term efficacy, lower recurrence and metastasis rate, higher survival rate and longer mean survival time. Compared with patients with GG genotype, those with GSTP1 AA + AG genotype also enjoyed more positive results. Our results reveal that GSTT1 null genotype and GSTP1 AA + AG genotype are associated with short- and long-term efficacy of CCRT in advanced OC..

Keywords: advanced ovarian cancer; GSTT1; GSTP1; GSTM1; Genetic polymorphisms; Concurrent chemoradiotherapy.

DOI: 10.37813/j.mbn.2707-4692.009

Published on: 12 October 2020

Viewed: 213 / Downloaded: 870 / Cited:0

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Mechanism of Long Non-Coding RNAs in Autophagy and Inflammation of Vascular Endothelial Cells: A Review

Li Peng;Kun Wu;

Abstract: Increasing researches have highlighted the properties of long non-coding RNAs (lncRNAs) regulating gene transcription, epigenetics, stem cells differentiation and other biological processes. Vascular endothelial cells have been implicated in the process of angiogenesis since they comprise the morphological basis of cardiovascular system. Vascular endothelial cell dysfunction in vascular endothelial cells affects the occurrence and progression of various cardiovascular diseases while secretion of inflammatory factors can induce vascular endothelial cells dysfunction. Autophagy has been widely regarded as an inflammation-related defensive mechanism in cardiovascular diseases. Emerging studies have addressed the involvement of lncRNAs in vascular endothelial cells. As vascular endothelia cells exert great effects on the cardiovascular system, this review discusses the correlations between lncRNAs and vascular endothelia cells with emphasis on regulation of lncRNAs on both autophagy and inflammation in vascular endothelia cells..

Keywords: long non-coding RNAs; vascular endothelial cells; autophagy; inflammation; mechanism; review.

DOI: 10.37813/j.mbn.2707-4692.008

Published on: 14 September 2020

Viewed: 81 / Downloaded: 783 / Cited:0

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